Sunday, 29 July 2012

HPLC-MS

In HPLC-MS (High Performance Liquid Chromatography), the components in the analyte are analyzed through the use of a Mass Spectrometer detector.

A Mass Spectrometer is a detector that measures the mass-to-charge ratio of the eluting components. This provides a very accurate identification of the components due to the high improbability of two different compounds of similar retention times having similar mass-to-charge ratios.

A drawback of the Mass Spectrometer detector is that it is a destructive detector, therefore the samples cannot be recovered after analysis.

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